E-ISSN 2683-5961 | ISSN 2354-1814
 
2023, Vol: 10, Issue: 2
10 / 2Current Issue Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Research Article

Online Publishing Date:
26 / 08 / 2022

 


Evaluation of Three Methods of DNA Extraction Using Fungi Samples

Rabiu Muhammad Aliyu, Abubakar Tanko, Abdulmalik Bello Shuaibu, mikaeel Bala Abubakar, Adamu Bello Kasarawa.


Abstract
Polymerase chain reaction-based assays have been developed to amplify DNA of fungal pathogens for
detection, identification and classification of fungi as culture-based methods show low sensitivity and
specificity. However, achieving a sensitive, specific, and reliable PCR-based assay depends on the availability
of qualitative DNA. A Simple and easy-to-perform DNA extraction protocol is essential. In this study, three
methods of DNA extraction were evaluated and compared in relation to their ability in the extraction of DNA
from fungal isolates in our laboratory. Qualitative (Purity) and quantitative evaluation of the extracted DNA
was based on spectrophotometric and electrophoretic techniques respectively. The results obtained from this
study indicated that, the Modified boiling (Tween 20) method was the most effective in obtaining fungal DNA
of high quality and quantity as compared to that of Cetyl Trimethyl Ammonium Bromide (CTAB) and Sodium
Dodecyl Sulfate (SDS) methods. Thus, we recommended the use of the modified boiling method (Tween 20)
for the extraction of genomic DNA from fungal isolates.

Key words: DNA extraction, Fungi, SDS, CTAB, Boiling method


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Rabiu Muhammad Aliyu
Articles by Abubakar Tanko
Articles by Abdulmalik Bello Shuaibu
Articles by mikaeel Bala Abubakar
Articles by Adamu Bello Kasarawa
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Aliyu RM, Tanko A, Shuaibu AB, Abubakar mB, Kasarawa AB. Evaluation of Three Methods of DNA Extraction Using Fungi Samples. Equijost. 2021; 8(2): 62-67. doi:https//dx.doi.org/10.4314/equijost.v8i2.11

Web Style

Aliyu RM, Tanko A, Shuaibu AB, Abubakar mB, Kasarawa AB. Evaluation of Three Methods of DNA Extraction Using Fungi Samples. https://www.equijost.com/?mno=83131 [Access: March 05, 2024]. doi:https//dx.doi.org/10.4314/equijost.v8i2.11

AMA (American Medical Association) Style

Aliyu RM, Tanko A, Shuaibu AB, Abubakar mB, Kasarawa AB. Evaluation of Three Methods of DNA Extraction Using Fungi Samples. Equijost. 2021; 8(2): 62-67. doi:https//dx.doi.org/10.4314/equijost.v8i2.11


Vancouver/ICMJE Style

Aliyu RM, Tanko A, Shuaibu AB, Abubakar mB, Kasarawa AB. Evaluation of Three Methods of DNA Extraction Using Fungi Samples. Equijost. (2021), [cited March 05, 2024]; 8(2): 62-67. doi:https//dx.doi.org/10.4314/equijost.v8i2.11


Harvard Style

Aliyu, R. M., Tanko, . A., Shuaibu, . A. B., Abubakar, . m. B. & Kasarawa, . A. B. (2021) Evaluation of Three Methods of DNA Extraction Using Fungi Samples. Equijost, 8 (2), 62-67. doi:https//dx.doi.org/10.4314/equijost.v8i2.11


Turabian Style

Aliyu, Rabiu Muhammad, Abubakar Tanko, Abdulmalik Bello Shuaibu, mikaeel Bala Abubakar, and Adamu Bello Kasarawa. 2021. Evaluation of Three Methods of DNA Extraction Using Fungi Samples. Equity Journal of Science and Technology, 8 (2), 62-67. doi:https//dx.doi.org/10.4314/equijost.v8i2.11


Chicago Style

Aliyu, Rabiu Muhammad, Abubakar Tanko, Abdulmalik Bello Shuaibu, mikaeel Bala Abubakar, and Adamu Bello Kasarawa. "Evaluation of Three Methods of DNA Extraction Using Fungi Samples." Equity Journal of Science and Technology 8 (2021), 62-67. doi:https//dx.doi.org/10.4314/equijost.v8i2.11


MLA (The Modern Language Association) Style

Aliyu, Rabiu Muhammad, Abubakar Tanko, Abdulmalik Bello Shuaibu, mikaeel Bala Abubakar, and Adamu Bello Kasarawa. "Evaluation of Three Methods of DNA Extraction Using Fungi Samples." Equity Journal of Science and Technology 8.2 (2021), 62-67. Print. doi:https//dx.doi.org/10.4314/equijost.v8i2.11


APA (American Psychological Association) Style

Aliyu, R. M., Tanko, . A., Shuaibu, . A. B., Abubakar, . m. B. & Kasarawa, . A. B. (2021) Evaluation of Three Methods of DNA Extraction Using Fungi Samples. Equity Journal of Science and Technology, 8 (2), 62-67. doi:https//dx.doi.org/10.4314/equijost.v8i2.11




Most Viewed Articles
Most Accessed Articles

  • RESPONSE OF COWPEA VARIETIES TO BASAL STEM ROT (Sclerotium Rolfsii) DISEASE IN SOUTHERN GUINEA SAVANNA, NIGERIA
    M. U. Tanimu, I. U. Mohammed, A. Muhammad and N.M. Kwaifa
    Equijost. 2018; 5(1): 1-8
    » Abstract

  • PATTERN AND DETERMINANTS OF CONSUMERS DEMAND FOR CANNED FOOD IN ILORIN METROPOLIS OF KWARA STATE, NIGERIA
    Babatunde, R.O.; Amolegbe, K.B.; Akingbohungbe, I.T.
    Equijost. 2018; 5(1): 23-34
    » Abstract

  • PREDICTION OF CUSTOMER ACCESSIBILITY OF ELECTRONIC BANKING LOGISTIC REGRESSION IN NIGERIA
    Suleiman, S. and Usman, U.
    Equijost. 2016; 4(1): 93-97
    » Abstract

  • EFFECT OF LATE PLANTING ON INCIDENCE AND SEVERITY OF STENOCARPELLA EAR ROT OF MAIZE IN LAFIA, NASARAWA STATE, NIGERIA
    Garba Y. and Ogara I. M.
    Equijost. 2016; 4(1): 57-60
    » Abstract

  • EFFECTS OF DIFFERENT HORMONES ON THE GROWTH PERFORMANCE OF Clarias gariepinus
    ADESHINA, I., ADEWALE, Y. A. and DALHATU, B. J.
    Equijost. 2016; 4(1): 10-13
    » Abstract

    • Most Downloaded
    Top Downloaded Articles

  • ROLE OF FADAMA III PROGRAMME IN EMPOWERING THE SOCIO-ECONOMIC STATUS OF THE SOKOTO STATE PARTICIPATING WOMEN THROUGH CROP PRODUCTIVE ASSETS
    Ango, A. K.; Maikasuwa, M. A.; Sidi, M. H.
    Equijost. 2018; 5(1): 14-22
    » Abstract

  • ELECTROMAGNETIC CHARACTERIZATION AND FIELD DISTRIBUTION OF CO0.5ZN0.5FE2O4 USING PATCH MICROSTRIP ANTENNA AND FINITE ELEMENT METHODS
    Abubakar Yakubu, Zulkifli Abbas, Dago M Mohammad
    Equijost. 2018; 5(1): 123-131
    » Abstract

  • Bacteria Contamination of Market Vended Ponmo (Processed Cow Hide) in Birnin Kebbi, Kebbi State
    Jibrin Naka Keta, Mohammed Naka Keta, Mubarak Aminu Aburga, Grace Joseph Gudu
    Equijost. 2020; 7(1): 41-45
    » Abstract

  • RELATIONSHIP BETWEEN BODY WEIGHT AND ZOOMETRIC MEASUREMENTS IN SOME PURE AND CROSSBRED TURKEY POULTS
    Amao, O. J., Ayorinde, K. L. and Fayeye, T. R.
    Equijost. 2016; 4(1): 24-30
    » Abstract

  • PHYSICOCHEMICAL ANALYSES OF SESAME (Sesamun indicum L.) SEED OIL AND SOAP PRODUCED FROM THE OIL
    Hassan, A.; Wawata, I.G.
    Equijost. 2018; 5(1): 162-167
    » Abstract

    • Most Cited Articles
    Most Cited Articles

    About Equity Journal of Science and Technology

    Equity Jornal of Scienec and Technology (EQUIJOST) is published by the Kebbi State University of Science and Technology, Aliero (KSUSTA) and supported by ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Equity Journal of Science and Technology

    Kebbi State University of Science and Technology, Aliero

    P. M. B. 1144, Kebbi State, Nigeria

    email: equijostjournal@gmail.com

    Tel: +2349038120169

     
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use