E-ISSN 2683-5961 | ISSN 2354-1814
 

Research Article 


Phytochemical, High Performance Liquid Chromatography (HPLC) and Antimicrobial Evaluations of the Ethanolic Root Extract of Phoenix dactylifera L.

Yakubu Yaaba, Shehu Busu Mohammed, Aliyu Adamu, Japhet Gaius Yakubu, 1Obi P. Adigwe.

Abstract
Bioactive compounds from plants have received a great interest from scientists all over the globe for development of drugs. This study was aimed at evaluating phytochemicals, High Performance Liquid Chromatography (HPLC) and antibacterial activities of ethanolic root extract of Phoenix dactylifera L. (date plant) against some clinical isolates. Roots of date plant were dried and extracted with ethanol using the cold maceration method before concentrating it with water bath at 45oC. The phytochemicals were identified using HPLC based on their individual retention time. Antibacterial activities of date extract were assessed against some clinical isolates including; Staphylococcus aureus, Klebsiella pneumoniae, Streptococcus pyogenes, and Pseudomonas aeruginosa utilizing agar disc diffusion method. The percentage yield of ethanol extract was 0.3892%. The result of the phytochemical screening revealed the presence of carbohydrate, phenols, tannins, saponins, flavonoids, cardiac glycosides, steroids, alkaloids and terpenes at various retention time of 2.853, 3.235, 4.129, 4.713, 6.722, 8.954, 11.228 and 22.912 minutes. Compounds identified include phenolic acid (caffeic acid) and two flavonoids (rutin and quercetin). The zone of inhibition diameter ranged from 15 to 19 mm. The maximum zone of inhibition was detected against Streptococcus pyogenes, (19mm). Staphylococcus aureus and Klebsiella pneumoniae were inhibited with 17mm and 16mm zone of inhibition respectively, followed by Pseudomonas aeruginosa which had the least zone of inhibition (15mm). In conclusion, the root of Phoenix dactylifera L. possesses some important phytochemical components with antibacterial activity that could be possibly exploited for pharmaceutical development.

Key words: Phytochemicals, Antibacterial activities, ethanolic root extract, Clinical isolates, Phoenix dactylifera L.


 
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How to Cite this Article
Pubmed Style

Yaaba Y, Mohammed SB, Adamu A, Yakubu JG, Adigwe OP. Phytochemical, High Performance Liquid Chromatography (HPLC) and Antimicrobial Evaluations of the Ethanolic Root Extract of Phoenix dactylifera L..


Web Style

Yaaba Y, Mohammed SB, Adamu A, Yakubu JG, Adigwe OP. Phytochemical, High Performance Liquid Chromatography (HPLC) and Antimicrobial Evaluations of the Ethanolic Root Extract of Phoenix dactylifera L.. http://www.equijost.com/?mno=134076 [Access: July 08, 2021].


AMA (American Medical Association) Style

Yaaba Y, Mohammed SB, Adamu A, Yakubu JG, Adigwe OP. Phytochemical, High Performance Liquid Chromatography (HPLC) and Antimicrobial Evaluations of the Ethanolic Root Extract of Phoenix dactylifera L..



Vancouver/ICMJE Style

Yaaba Y, Mohammed SB, Adamu A, Yakubu JG, Adigwe OP. Phytochemical, High Performance Liquid Chromatography (HPLC) and Antimicrobial Evaluations of the Ethanolic Root Extract of Phoenix dactylifera L..



Harvard Style

Yaaba, Y., Mohammed, . S. B., Adamu, . A., Yakubu, . J. G. & Adigwe, . O. P. (0) Phytochemical, High Performance Liquid Chromatography (HPLC) and Antimicrobial Evaluations of the Ethanolic Root Extract of Phoenix dactylifera L..



Turabian Style

Yaaba, Yakubu, Shehu Busu Mohammed, Aliyu Adamu, Japhet Gaius Yakubu, and Obi P. Adigwe. 0. Phytochemical, High Performance Liquid Chromatography (HPLC) and Antimicrobial Evaluations of the Ethanolic Root Extract of Phoenix dactylifera L..



Chicago Style

Yaaba, Yakubu, Shehu Busu Mohammed, Aliyu Adamu, Japhet Gaius Yakubu, and Obi P. Adigwe. "Phytochemical, High Performance Liquid Chromatography (HPLC) and Antimicrobial Evaluations of the Ethanolic Root Extract of Phoenix dactylifera L.."



MLA (The Modern Language Association) Style

Yaaba, Yakubu, Shehu Busu Mohammed, Aliyu Adamu, Japhet Gaius Yakubu, and Obi P. Adigwe. "Phytochemical, High Performance Liquid Chromatography (HPLC) and Antimicrobial Evaluations of the Ethanolic Root Extract of Phoenix dactylifera L.."



APA (American Psychological Association) Style

Yaaba, Y., Mohammed, . S. B., Adamu, . A., Yakubu, . J. G. & Adigwe, . O. P. (0) Phytochemical, High Performance Liquid Chromatography (HPLC) and Antimicrobial Evaluations of the Ethanolic Root Extract of Phoenix dactylifera L..





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