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Research Article
Online Published: 21 Sep 2024
 


Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase

Bamidele Sanya Fagbohunka, Olamide Tolulope Sowunmi, Olusola Tosin Lawal, Kuburat Temitope Odufuwa, Akinkunmi Apena, Muinat Morounke Adeyanju.


Abstract
One of the lignocellulolytic and hydrolytic enzymes needed for a number of processes is cellulase. The biochemical characteristics of pure cellulase derived from Oryctes rhinoceros were documented in this work. Following the extraction of the precipitant from the crude cellulase generated by ammonium sulphate, the enzyme purity was ensured by gel filtration and affinity chromatography employing Reactive Blue 2-Agarose resins and Biogel P-100 resins, respectively. With a 15% yield and 3.59 U/mg of specific activity, the purified cellulase displayed a purification fold of 10.2. The isolated cellulase showed peak activity at pH 4.0 and 70°C, with a native molecular weight of 54,300daltons. Using glucose as the substrate, the Vmax and Km of the isolated enzyme were deduced as 50µmol/min/ml and 5.0mg/ml respectively. The enzyme's broad substrate specificity was demonstrated by the addition of other carbohydrates to the enzyme assay. Certain metal ions and substances like urea, 2-mercaptoethanol, and EDTA inhibited inhibit the enzymatic activity. The gut cellulase of Rhinoceros larvae has shown properties that are useful in biotechnological and industrial operations.

Key words: Characterization, metabisulfite, Oryctes rhinoceros, purification, tetraborate


 
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How to Cite this Article
Pubmed Style

Fagbohunka BS, Sowunmi OT, Lawal OT, Odufuwa KT, Apena A, Adeyanju MM. Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase. Equijost. 2024; 11(1): 22-28. doi:10.5455/EQUIJOST.159958

Web Style

Fagbohunka BS, Sowunmi OT, Lawal OT, Odufuwa KT, Apena A, Adeyanju MM. Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase. https://www.equijost.com/?mno=159958 [Access: June 25, 2025]. doi:10.5455/EQUIJOST.159958

AMA (American Medical Association) Style

Fagbohunka BS, Sowunmi OT, Lawal OT, Odufuwa KT, Apena A, Adeyanju MM. Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase. Equijost. 2024; 11(1): 22-28. doi:10.5455/EQUIJOST.159958


Vancouver/ICMJE Style

Fagbohunka BS, Sowunmi OT, Lawal OT, Odufuwa KT, Apena A, Adeyanju MM. Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase. Equijost. (2024), [cited June 25, 2025]; 11(1): 22-28. doi:10.5455/EQUIJOST.159958


Harvard Style

Fagbohunka, B. S., Sowunmi, . O. T., Lawal, . O. T., Odufuwa, . K. T., Apena, . A. & Adeyanju, . M. M. (2024) Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase. Equijost, 11 (1), 22-28. doi:10.5455/EQUIJOST.159958


Turabian Style

Fagbohunka, Bamidele Sanya, Olamide Tolulope Sowunmi, Olusola Tosin Lawal, Kuburat Temitope Odufuwa, Akinkunmi Apena, and Muinat Morounke Adeyanju. 2024. Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase. Equity Journal of Science and Technology, 11 (1), 22-28. doi:10.5455/EQUIJOST.159958


Chicago Style

Fagbohunka, Bamidele Sanya, Olamide Tolulope Sowunmi, Olusola Tosin Lawal, Kuburat Temitope Odufuwa, Akinkunmi Apena, and Muinat Morounke Adeyanju. "Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase." Equity Journal of Science and Technology 11 (2024), 22-28. doi:10.5455/EQUIJOST.159958


MLA (The Modern Language Association) Style

Fagbohunka, Bamidele Sanya, Olamide Tolulope Sowunmi, Olusola Tosin Lawal, Kuburat Temitope Odufuwa, Akinkunmi Apena, and Muinat Morounke Adeyanju. "Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase." Equity Journal of Science and Technology 11.1 (2024), 22-28. Print. doi:10.5455/EQUIJOST.159958


APA (American Psychological Association) Style

Fagbohunka, B. S., Sowunmi, . O. T., Lawal, . O. T., Odufuwa, . K. T., Apena, . A. & Adeyanju, . M. M. (2024) Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase. Equity Journal of Science and Technology, 11 (1), 22-28. doi:10.5455/EQUIJOST.159958




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