Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase

Bamidele Sanya Fagbohunka; Olamide Tolulope Sowunmi; Olusola Tosin Lawal; Kuburat Temitope Odufuwa; Akinkunmi Apena; Muinat Morounke Adeyanju

doi:10.5455/EQUIJOST.159958

2024, Vol: 11, Issue: 2

11 / 2Current Issue Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

« Previous Article

Research Article
Online Published: 21 Sep 2024

Equijost. 2024; 11(1): 22-28

doi: 10.5455/EQUIJOST.159958

Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase

Bamidele Sanya Fagbohunka, Olamide Tolulope Sowunmi, Olusola Tosin Lawal, Kuburat Temitope Odufuwa, Akinkunmi Apena, Muinat Morounke Adeyanju.

Abstract
One of the lignocellulolytic and hydrolytic enzymes needed for a number of processes is cellulase. The biochemical characteristics of pure cellulase derived from Oryctes rhinoceros were documented in this work. Following the extraction of the precipitant from the crude cellulase generated by ammonium sulphate, the enzyme purity was ensured by gel filtration and affinity chromatography employing Reactive Blue 2-Agarose resins and Biogel P-100 resins, respectively. With a 15% yield and 3.59 U/mg of specific activity, the purified cellulase displayed a purification fold of 10.2. The isolated cellulase showed peak activity at pH 4.0 and 70°C, with a native molecular weight of 54,300daltons. Using glucose as the substrate, the Vmax and Km of the isolated enzyme were deduced as 50µmol/min/ml and 5.0mg/ml respectively. The enzyme's broad substrate specificity was demonstrated by the addition of other carbohydrates to the enzyme assay. Certain metal ions and substances like urea, 2-mercaptoethanol, and EDTA inhibited inhibit the enzymatic activity. The gut cellulase of Rhinoceros larvae has shown properties that are useful in biotechnological and industrial operations.

Key words: Characterization, metabisulfite, Oryctes rhinoceros, purification, tetraborate

	ARTICLE TOOLS
	Abstract
	PDF Fulltext
	How to cite this article
	Citation Tools
	Related Records
	Articles by Bamidele Sanya Fagbohunka Articles by Olamide Tolulope Sowunmi Articles by Olusola Tosin Lawal Articles by Kuburat Temitope Odufuwa Articles by Akinkunmi Apena Articles by Muinat Morounke Adeyanju
	on Google
	on Google Scholar

How to Cite this Article

Pubmed Style

Fagbohunka BS, Sowunmi OT, Lawal OT, Odufuwa KT, Apena A, Adeyanju MM. Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase. Equijost. 2024; 11(1): 22-28. doi:10.5455/EQUIJOST.159958

Web Style

Fagbohunka BS, Sowunmi OT, Lawal OT, Odufuwa KT, Apena A, Adeyanju MM. Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase. https://www.equijost.com/?mno=159958 [Access: June 25, 2025]. doi:10.5455/EQUIJOST.159958

AMA (American Medical Association) Style

Fagbohunka BS, Sowunmi OT, Lawal OT, Odufuwa KT, Apena A, Adeyanju MM. Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase. Equijost. 2024; 11(1): 22-28. doi:10.5455/EQUIJOST.159958

Vancouver/ICMJE Style

Fagbohunka BS, Sowunmi OT, Lawal OT, Odufuwa KT, Apena A, Adeyanju MM. Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase. Equijost. (2024), [cited June 25, 2025]; 11(1): 22-28. doi:10.5455/EQUIJOST.159958

Harvard Style

Fagbohunka, B. S., Sowunmi, . O. T., Lawal, . O. T., Odufuwa, . K. T., Apena, . A. & Adeyanju, . M. M. (2024) Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase. Equijost, 11 (1), 22-28. doi:10.5455/EQUIJOST.159958

Turabian Style

Fagbohunka, Bamidele Sanya, Olamide Tolulope Sowunmi, Olusola Tosin Lawal, Kuburat Temitope Odufuwa, Akinkunmi Apena, and Muinat Morounke Adeyanju. 2024. Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase. Equity Journal of Science and Technology, 11 (1), 22-28. doi:10.5455/EQUIJOST.159958

Chicago Style

Fagbohunka, Bamidele Sanya, Olamide Tolulope Sowunmi, Olusola Tosin Lawal, Kuburat Temitope Odufuwa, Akinkunmi Apena, and Muinat Morounke Adeyanju. "Purification and Biochemical Characterization of a purified Oryctes Rhinoceros Larva Cellulase." Equity Journal of Science and Technology 11 (2024), 22-28. doi:10.5455/EQUIJOST.159958

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

About Equity Journal of Science and Technology

Equity Jornal of Scienec and Technology (EQUIJOST) is published by the Kebbi State University of Science and Technology, Aliero (KSUSTA) and supported by ... Read more.

For best results, please use Internet Explorer or Google Chrome.

Contact Information

Equity Journal of Science and Technology

Kebbi State University of Science and Technology, Aliero

P. M. B. 1144, Kebbi State, Nigeria

email: equijostjournal@gmail.com

Tel: +2349038120169

POLICIES & JOURNAL LINKS

Author Login
Reviewer Login
Author Login Reviewer Login Editorial Policies

About Equity Journal of Science and Technology

Contact Information

How to cite this article